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Image Search Results
Journal: Polymers
Article Title: Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer
doi: 10.3390/polym11091391
Figure Lengend Snippet: Functional in vivo chondrogenesis of hASCs. ( a ) The presence of glycosaminoglycans and collagen demonstrated chondrogenic differentiation by Alcian Blue and Masson’s Thrichrome staining, separately. Alcian Blue, Nuclear Fast Red, and Masson’s Thrichrome staining of the xenografts from ECM-hASCs-glue and ECM-glue groups three months afterwards. Upper panel represents the ECM-glue group. Lower panel represents the ECM-hASCs-glue group. Masson’s Trichrome staining of the ECM-hASCs-glue and ECM-glue groups after three months implantation (Original magnification × 200). (Arrows indicate the interstitial spaces.) ( b ) Immunofluorescent staining for Aggrecan for identifying glycosaminoglycan (green) and FND-labeled hASCs (red) in ECM-hASCs-glue and ECM-glue groups. ( c ) Fluorescence imaging of type II collagen (green) were presented in ECM-hASCs-glue and ECM-glue groups. Scale bar: 50 μm.
Article Snippet: The cells were then induced to adipogenic, osteogenic, or chondrogenic differentiation by using hMSC adipogenic induction SingleQuotsTM kit (Lonza PT-4135, Bazel, Switzerland) or a hMSC osteogenic SingleQuotsTM kit (Lonza PT-4120, Bazel, Switzerland) or
Techniques: Functional Assay, In Vivo, Staining, Labeling, Fluorescence, Imaging